DNA vaccines : methods and protocols
In the early 1990s, almost 200 yr after Edward Jenner demonstrated the effectiveness of the smallpox vaccine, a new paradigm for vaccination emerged. The conventional method of vaccination required delivery of whole pathogens or structural subunits, but in this new approach, DNA or genetic information was administered to elicit an immunological response. Once it was observed that plasmid DNA delivered in vivo led to production of an encoded transgene (1), two ground-breaking studies demonstrated that immunological responses could be generated against antigenic transgenes via plasmid DNA delivered by DNA vaccination (as this approach is called) (2,3). The appe- ance of this new vaccination strategy coincided with advances in molecular biology, which provided new tools to study and manipulate the basic elements of an organism's genome and also could also be applied to the design and production of DNA vaccines. DNA Vaccines is a major updated and enhancement of the first edition. It reviews state-of-the-art methods in DNA vaccine technology, with chapters describing DNA vaccine design, delivery systems, adjuvants, current appli- tions, methods of production, and quality control. Consistent with the approach of the Methods in Molecular Medicine series, these chapters contain detailed practical procedures on the latest DNA vaccine technology. The enthusiasm for DNA vaccine technology is made clear by the number of research studies published on this topic since the mid-1990s
Laboratory Manual
1 online resource (xv, 384 pages) : illustrations
9781588294845, 9781597451680, 9781280832529, 9786610832521, 1588294846, 1597451681, 1280832525, 6610832528
209947559
DNA vaccine design / Janet L. Brandsma
Design of plasmid DNA constructs for vaccines / Donna L. Montgomery and Kristala Jones Prather
Vaccination with messenger RNA / Stave Pascolo
A stress protein-facilitated antigen expression system for plasmid DNA vaccines / Petra Riedl [and others]
In vitro assay of immunostimulatory activities of plasmid vectors / Weiwn Jiang, Charles F. Reich, and David S. Pisetsky
Delivery of DNA vaccines using electroporation / Shawn Babiuk, Sylvia van Drunen Littel-van den Hurk, and Lorne A. Babiuk
Needle-free injection of DNA vaccines: a brief overview and methodology / Kanakatte Raviprakash and Kevin R. Porter
Needle-free delivery of veterinary DNA vaccines / Sylvia van Drunen Littel-van den Hurk, Shawn Babiuk, and Lorne A. Babiuk
Surface-modified biodegradable microspheres for DNA vaccine delivery / Mark E. Keegan and W. Mark Saltzman
A dendrimer-like DNA-based vector for DNA delivery: a viral and nonviral hybrid approach / Dan Luo [and others]
Identification of compartments involved in mammalian subcellular trafficking pathways by indirect immunofluorescence / Anne Doody and David Putnam
Adjuvant properties of CpG oligonucleotides in primates / Daniela Verthelyi
Complexes of DNA vaccines with cationic, antigenic peptides are potent, polyvalent CD8+ T-cell stimulating immunogens / Petra Riedl, Jörg Reimann, and Reinhold Schirmbeck
Prime-boost strategies in DNA vaccines / C. Jane Dale [and others]
Modifying professional antigen-presenting cells to enhance DNA vaccine potency / Chien-Fu Hung, Mu Yang, and T.C. Wu
Replicase-based DNA vaccines for allergy treatment / Sandra Scheiblhofer [and others]
Immunological responses of neonates and infants to DNA vaccines / Martha Sedegah and Stepen L. Hoffman
DNA vaccines for allergy treatment / Richard Weiss, Sandra Sheiblhofer, and Josef Thalhamer
Protection from autoimmunity by DNA vaccination against T-cell receptor / Thorsten Buch and Ari Waisman
The use of bone marrow-chimeric mice in elucidating immune mechanisms / Akiko Iwasaki
A simple method for the production of plasmid DNA in bioreactors / Kristin Listner, Laura Kizer Bentley, and Michel Chartrain
Practical methods for supercoiled pDNA production / John Ballantyne
Production of plasmid DNA in industrial quantities according to cGMP guidelines / Joachim Schorr [and others]
Large-scale, nonchromatographic purification of plasmid DNA / Jason C. Murphy, Michael A. Winters, and Sangeetha L. Sagar
Assuring the quality, safety, and efficacy of DNA vaccines / James S. Robertson and Elwyn Griffiths
English
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