tion is prepared by mixing 0.6 ml of a stock solution with 5,000 ml of distilled water. The stock solution contains 24 grams of active material per 100 ml of propylene glycol or two pounds of active material per gallon. A solution which is five times this concentration (5 x 29 ppm) may be used for extremely dormant seeds, provided seeds are transferred to substratum moistened with water after 1 to 3 days.

(11) Ethylene. This term means that five (5) ml of ethylene gas per cubic foot (176.57 ml/m3) of germinator space is injected into a germinator in which peanut seeds in moist rolled towels have been placed. Following injection of the ethylene, the germinator is kept closed until the first count (5 days). If the germinator door is opened for the purpose of checking or rewetting the samples, another injection of ethylene at the same rate shall be made.

(b) Special procedures and alternate methods for germination referred to in table 2-(1) Alyce clover (Alysicarpusvaginalis); Swollen seeds. At the conclusion of the 21-day test period carefully pierce the seedcoat with a sharp instrument and continue the test for 5 additional days.

Alternate method: The swollen seeds may be placed at 20° C. for 48 hours and then at 35° C. for 3 additional days.

(2) Bahiagrass (Paspalum notatum); removal of glumes. On all varieties except "Pensacola", remove the enclosing structures (glumes, lemma, and palea) from the caryopsis with the aid of a sharp scalpel. It the seed is fresh or dormant, scratch the surface of the caryopsis lightly.

(3) Beet, Swiss chard (Beta); preparation of seed for test. Before placing the seeds on the germination substratum they shall be soaked in water for 2 hours, using at least 250 ml. of water per 100 seeds, then washed in running water and the excess water should be blotted off. The temperature of the soaking and washing water should be no lower than 20° C. Samples producing darkened radicles should be retested in soil or by washing in running water for 3 hours and tested "Kimpak," keeping the seed covered with slightly moist blotters. Sugar beets may require 16 hours soaking in

on

water at 25° C., followed by rinsing and then drying for 2 hours at room temperature.

(4) Buffelgrass (Pennisetum ciliare); alternate method for dormant seed. The caryopses shall be removed from the fascicles and placed on blotters moistened with a 0.2 percent potassium nitrate solution, in Petri dishes. The seeds from a fascicle should be arranged so they will not be confused with seeds from other fascicles during the test. The seeds are then prechilled at 5° C. for 7 days and tested at 30° C. in light for 21 additional days. Firm ungerminated seeds remaining at the conclusion of the test should be scratched lightly and left in test for 7 additional days.

(5) Cotton (Gossypium spp.); dormant samples. Samples of cottonseed which do not respond to the usual method should be placed in a closed container with water and shaken until the lint is thoroughly wet. The excess moisture should then be blotted off.

(6) Endive (Cichorium endivia); dormant samples. Add about % inch of tap water at the beginning of the test and remove excess water after 24 hours.

(7) [Reserved]

(8) Rescue grass (Bromus catharticus); dormant samples. Wash for 48 hours in running water, or soak for 48 hours, changing the water and rinsing each morning and night.

(9) Rice (Oryza sativa)—Alternate method. Plant the seeds in moist sand. On the seventh day of the test add water to a depth of one-fourth inch above the sand level and leave for the remainder of the test. Only a final count is made. Dormant seeds: Presoak 24 to 48 hours in 40° C. water. For deeply dormant seeds, presoak 24 hours in 1,000 p.p.m. ethylene chlorohydrin or 5 percent solution of sodium hypochlorite at bottle strength).

(clorox

(10) Ryegrass (Lolium); fluorescencetest. The germination test for the fluorescence of ryegrass shall be conducted in light (not to exceed 100 foot-canIdles) with white filter paper as a substratum. The test shall be conducted in a manner that will prevent the contact of roots of different seedlings. If there are over 75 percent normal fluo

rescent seedlings present at the time of the first count, break the contact of the roots of the nonfluorescent seedlings from the substratum and reread the fluorescence at the time of the final count.

(11) Trifolium, Medicago, Melilotus, and Vicia faba; temperature requirements. A temperature of 18° C. is desirable for Trifolium spp., Medicago spp., Melilotus spp., and Vicia faba.

(12) Garden beans (Phaseolus vulgaris); use of calcium nitrate. If hypocotyl collar rot is observed on seed

lings, the sample involved may be retested using a 0.3 to 0.6 percent solution of calcium nitrate to moisten the germination medium.

(13) Fourwing Saltbush (Atriplex canescens); preparation of seed for test. De-wing seeds and soak for 2 hours in 3 liters of water after which rinse with approximately 3 liters of distilled water. Remove excess water, air dry for 7 days at room temperature, then test for germination as indicated in Table 2.

20-35

30

14 Light; KNO,.

Prechill at 5° C. for 6 weeks; test 14
additional days. See Dormant seeds-
§ 201.57a.

See Dormant seeds-§ 201.57a.

28 Light; press fascicles into well-packed soil See par. (b)(4). See Dormant seedsand prechill at 5° C. for 7 days.

§ 201.57a.

20

20