DNA VaccinesMark W. Saltzman, Hong Shen, Janet L. Brandsma Springer Science & Business Media, 2008 M02 2 - 384 pages In the early 1990s, almost 200 yr after Edward Jenner demonstrated the effectiveness of the smallpox vaccine, a new paradigm for vaccination emerged. The conventional method of vaccination required delivery of whole pathogens or structural subunits, but in this new approach, DNA or genetic information was administered to elicit an immunological response. Once it was observed that plasmid DNA delivered in vivo led to production of an encoded transgene (1), two ground-breaking studies demonstrated that immunological responses could be generated against antigenic transgenes via plasmid DNA delivered by DNA vaccination (as this approach is called) (2,3). The appe- ance of this new vaccination strategy coincided with advances in molecular biology, which provided new tools to study and manipulate the basic elements of an organism’s genome and also could also be applied to the design and production of DNA vaccines. DNA Vaccines is a major updated and enhancement of the first edition. It reviews state-of-the-art methods in DNA vaccine technology, with chapters describing DNA vaccine design, delivery systems, adjuvants, current appli- tions, methods of production, and quality control. Consistent with the approach of the Methods in Molecular Medicine series, these chapters contain detailed practical procedures on the latest DNA vaccine technology. The enthusiasm for DNA vaccine technology is made clear by the number of research studies published on this topic since the mid-1990s. |
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Page v
... yield useful vaccines for a large number of infectious diseases and have made only modest progress in treating cancer. DNA vaccines may be able to engage immunological mechanisms that are not easily attainable with other approaches ...
... yield useful vaccines for a large number of infectious diseases and have made only modest progress in treating cancer. DNA vaccines may be able to engage immunological mechanisms that are not easily attainable with other approaches ...
Page 11
... yields of plas- mid, preferably through a simple production process. For this reason, bacterial plasmids propagated in ... yield of plasmid molecules to meet the commercial needs, given that the issues of potential integration into the ...
... yields of plas- mid, preferably through a simple production process. For this reason, bacterial plasmids propagated in ... yield of plasmid molecules to meet the commercial needs, given that the issues of potential integration into the ...
Page 12
... yield between 500 and 700 copies per bacterial cell and are readily available as a result of their widespread use ... yields from the fermentation process , although the copy number can be increased by manipu- lating the growth rate of ...
... yield between 500 and 700 copies per bacterial cell and are readily available as a result of their widespread use ... yields from the fermentation process , although the copy number can be increased by manipu- lating the growth rate of ...
Page 17
... time ) , achieve a high cell density , and give the highest plasmid DNA yields in the fermentation medium in large - scale production . 1. Transform multiple strains of E. coli with a 1/100 Plasmid DNA Construct Design 17.
... time ) , achieve a high cell density , and give the highest plasmid DNA yields in the fermentation medium in large - scale production . 1. Transform multiple strains of E. coli with a 1/100 Plasmid DNA Construct Design 17.
Page 18
... yield of plasmid DNA from each set of strains . This can be done qualitatively by comparing the band intensities of plasmid DNA minipreps iso- lated from each strain following agarose gel electrophoresis . Alternatively , the DNA ...
... yield of plasmid DNA from each set of strains . This can be done qualitatively by comparing the band intensities of plasmid DNA minipreps iso- lated from each strain following agarose gel electrophoresis . Alternatively , the DNA ...
Contents
11 | |
A Stress ProteinFacilitated Antigen Expression System | 41 |
Weiwen Jiang Charles F Reich and David S Pisetsky | 55 |
Delivery of DNA Vaccines Using Electroporation | 73 |
and Methodology | 83 |
Sylvia van Drunen Littelvan den Hurk Shawn Babiuk | 91 |
for DNA Vaccine Delivery | 107 |
Subcellular Trafficking Pathways by Indirect | 127 |
Sandra Scheiblhofer Richard Weiss Maximilian Gabler | 221 |
Immunological Responses of Neonates and Infants | 239 |
DNA Vaccines for Allergy Treatment | 253 |
Protection From Autoimmunity by DNA Vaccination | 269 |
Immune Mechanisms | 281 |
DNA VACCINE PRODUCTION PURIFICATION AND QUALITY | 293 |
Production of Plasmid DNA in Industrial Quantities According | 339 |
LargeScale Nonchromatographic Purification of Plasmid | 351 |
Adjuvant Properties of CpG Oligonucleotides in Primates | 139 |
Complexes of DNA Vaccines With Cationic Antigenic Peptides | 159 |
PrimeBoost Strategies in DNA Vaccines | 171 |
Modifying Professional AntigenPresenting Cells to Enhance | 199 |
Assuring the Quality Safety and Efficacy of DNA Vaccines | 363 |
Index | 375 |
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Common terms and phrases
acid activation adjuvants antibody antigen antigen-specific APCs approx assay bacterial DNA Biojector buffer cationic CD8+ T cells cellular centrifuge chromatography clinical trials cloning codon coli concentration conjugation containing cover slips CpG motifs CpG ODN culture cytokine delivery dendritic cells detection diluted DL-DNA DNA vaccines electroporation ELISPOT encoding endotoxin enhance enzyme epitopes ethanol expression filter g/mL gene gun genomic human immune responses immunogenicity Immunol immunostimulatory Incubate induce injection Invitrogen lysate lysis macaques medium Methods and Protocols MHC class mice microspheres Molecular molecules mouse mRNA needle-free neonates Note oligodeoxynucleotides oligonucleotides optimal PBMC pDNA pellet peptide plasmid DNA plate PLGA Prepare priming production protein purification Qiagen receptor recombinant Resuspend room temperature RPMI sample sequence serum solution specific sterile stimulation strategies supernatant syringe T-cell T-cell responses target tion tissue transfected tube tumor vector viral virus vitro vivo Wash µg/mL