DNA Vaccines

Front Cover
Mark W. Saltzman, Hong Shen, Janet L. Brandsma
Springer Science & Business Media, 2008 M02 2 - 384 pages
In the early 1990s, almost 200 yr after Edward Jenner demonstrated the effectiveness of the smallpox vaccine, a new paradigm for vaccination emerged. The conventional method of vaccination required delivery of whole pathogens or structural subunits, but in this new approach, DNA or genetic information was administered to elicit an immunological response. Once it was observed that plasmid DNA delivered in vivo led to production of an encoded transgene (1), two ground-breaking studies demonstrated that immunological responses could be generated against antigenic transgenes via plasmid DNA delivered by DNA vaccination (as this approach is called) (2,3). The appe- ance of this new vaccination strategy coincided with advances in molecular biology, which provided new tools to study and manipulate the basic elements of an organism’s genome and also could also be applied to the design and production of DNA vaccines. DNA Vaccines is a major updated and enhancement of the first edition. It reviews state-of-the-art methods in DNA vaccine technology, with chapters describing DNA vaccine design, delivery systems, adjuvants, current appli- tions, methods of production, and quality control. Consistent with the approach of the Methods in Molecular Medicine series, these chapters contain detailed practical procedures on the latest DNA vaccine technology. The enthusiasm for DNA vaccine technology is made clear by the number of research studies published on this topic since the mid-1990s.

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Contents

Montgomery and Kristala Jones Prather 3 Vaccination With Messenger RNA
11
A Stress ProteinFacilitated Antigen Expression System
41
Weiwen Jiang Charles F Reich and David S Pisetsky
55
Delivery of DNA Vaccines Using Electroporation
73
and Methodology
83
Sylvia van Drunen Littelvan den Hurk Shawn Babiuk
91
for DNA Vaccine Delivery
107
Subcellular Trafficking Pathways by Indirect
127
Sandra Scheiblhofer Richard Weiss Maximilian Gabler
221
Immunological Responses of Neonates and Infants
239
DNA Vaccines for Allergy Treatment
253
Protection From Autoimmunity by DNA Vaccination
269
Immune Mechanisms
281
DNA VACCINE PRODUCTION PURIFICATION AND QUALITY
293
Production of Plasmid DNA in Industrial Quantities According
339
LargeScale Nonchromatographic Purification of Plasmid
351

Adjuvant Properties of CpG Oligonucleotides in Primates
139
Complexes of DNA Vaccines With Cationic Antigenic Peptides
159
PrimeBoost Strategies in DNA Vaccines
171
Modifying Professional AntigenPresenting Cells to Enhance
199
Assuring the Quality Safety and Efficacy of DNA Vaccines
363
Index
375
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