DNA VaccinesMark W. Saltzman, Hong Shen, Janet L. Brandsma Springer Science & Business Media, 2008 M02 2 - 384 pages In the early 1990s, almost 200 yr after Edward Jenner demonstrated the effectiveness of the smallpox vaccine, a new paradigm for vaccination emerged. The conventional method of vaccination required delivery of whole pathogens or structural subunits, but in this new approach, DNA or genetic information was administered to elicit an immunological response. Once it was observed that plasmid DNA delivered in vivo led to production of an encoded transgene (1), two ground-breaking studies demonstrated that immunological responses could be generated against antigenic transgenes via plasmid DNA delivered by DNA vaccination (as this approach is called) (2,3). The appe- ance of this new vaccination strategy coincided with advances in molecular biology, which provided new tools to study and manipulate the basic elements of an organism’s genome and also could also be applied to the design and production of DNA vaccines. DNA Vaccines is a major updated and enhancement of the first edition. It reviews state-of-the-art methods in DNA vaccine technology, with chapters describing DNA vaccine design, delivery systems, adjuvants, current appli- tions, methods of production, and quality control. Consistent with the approach of the Methods in Molecular Medicine series, these chapters contain detailed practical procedures on the latest DNA vaccine technology. The enthusiasm for DNA vaccine technology is made clear by the number of research studies published on this topic since the mid-1990s. |
From inside the book
Results 1-5 of 79
Page ii
... Assays , edited by Rosalyn D. Blumethal , 2005 109. Adoptive Immunotherapy : Methods and Protocols , edited by Burkhard Ludewig and Matthias W. Hoffman , 2005 108. Hypertension : Methods and Protocols , edited by Jérôme P. Fennell and ...
... Assays , edited by Rosalyn D. Blumethal , 2005 109. Adoptive Immunotherapy : Methods and Protocols , edited by Burkhard Ludewig and Matthias W. Hoffman , 2005 108. Hypertension : Methods and Protocols , edited by Jérôme P. Fennell and ...
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... Assay of Immunostimulatory Activities of Plasmid Vectors Weiwen Jiang, Charles F. Reich, and David S. Pisetsky ..................... PART II. DNA VACCINE DELIVERY SYSTEMS 6 Delivery of DNA Vaccines Using Electroporation Shawn Babiuk ...
... Assay of Immunostimulatory Activities of Plasmid Vectors Weiwen Jiang, Charles F. Reich, and David S. Pisetsky ..................... PART II. DNA VACCINE DELIVERY SYSTEMS 6 Delivery of DNA Vaccines Using Electroporation Shawn Babiuk ...
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... assayed in vitro. For example, the E2 protein of PV is a transactivating protein whose expression can be determined using an E2 pro- moter-containing ... assay (12). 3.4. Augmentation of Protein Expression From a DNA Vector (see 6 Brandsma.
... assayed in vitro. For example, the E2 protein of PV is a transactivating protein whose expression can be determined using an E2 pro- moter-containing ... assay (12). 3.4. Augmentation of Protein Expression From a DNA Vector (see 6 Brandsma.
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... assay reagent (Pierce). 13. 12% Tris-glycine gels (Novex). 14. Immobilon-P membranes (Millipore). 15. Western Breeze (Invitrogen). 16. Sodium dodecyl sulfate (SDS) polyacrylamide gel electrophoresis and transfer equipment. 2.4. Choosing ...
... assay reagent (Pierce). 13. 12% Tris-glycine gels (Novex). 14. Immobilon-P membranes (Millipore). 15. Western Breeze (Invitrogen). 16. Sodium dodecyl sulfate (SDS) polyacrylamide gel electrophoresis and transfer equipment. 2.4. Choosing ...
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... Assay Reagent ( Pierce ) and load equal amounts of total cell protein on 12 % Tris - glycine gels ( Novex ) . 6. Transfer to Immobilon - P membranes ( Millipore ) and detect the antigen expres- sion using Western Breeze ( Invitrogen ) ...
... Assay Reagent ( Pierce ) and load equal amounts of total cell protein on 12 % Tris - glycine gels ( Novex ) . 6. Transfer to Immobilon - P membranes ( Millipore ) and detect the antigen expres- sion using Western Breeze ( Invitrogen ) ...
Contents
11 | |
A Stress ProteinFacilitated Antigen Expression System | 41 |
Weiwen Jiang Charles F Reich and David S Pisetsky | 55 |
Delivery of DNA Vaccines Using Electroporation | 73 |
and Methodology | 83 |
Sylvia van Drunen Littelvan den Hurk Shawn Babiuk | 91 |
for DNA Vaccine Delivery | 107 |
Subcellular Trafficking Pathways by Indirect | 127 |
Sandra Scheiblhofer Richard Weiss Maximilian Gabler | 221 |
Immunological Responses of Neonates and Infants | 239 |
DNA Vaccines for Allergy Treatment | 253 |
Protection From Autoimmunity by DNA Vaccination | 269 |
Immune Mechanisms | 281 |
DNA VACCINE PRODUCTION PURIFICATION AND QUALITY | 293 |
Production of Plasmid DNA in Industrial Quantities According | 339 |
LargeScale Nonchromatographic Purification of Plasmid | 351 |
Adjuvant Properties of CpG Oligonucleotides in Primates | 139 |
Complexes of DNA Vaccines With Cationic Antigenic Peptides | 159 |
PrimeBoost Strategies in DNA Vaccines | 171 |
Modifying Professional AntigenPresenting Cells to Enhance | 199 |
Assuring the Quality Safety and Efficacy of DNA Vaccines | 363 |
Index | 375 |
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Common terms and phrases
acid activation adjuvants antibody antigen antigen-specific APCs approx assay bacterial DNA Biojector buffer cationic CD8+ T cells cellular centrifuge chromatography clinical trials cloning codon coli concentration conjugation containing cover slips CpG motifs CpG ODN culture cytokine delivery dendritic cells detection diluted DL-DNA DNA vaccines electroporation ELISPOT encoding endotoxin enhance enzyme epitopes ethanol expression filter g/mL gene gun genomic human immune responses immunogenicity Immunol immunostimulatory Incubate induce injection Invitrogen lysate lysis macaques medium Methods and Protocols MHC class mice microspheres Molecular molecules mouse mRNA needle-free neonates Note oligodeoxynucleotides oligonucleotides optimal PBMC pDNA pellet peptide plasmid DNA plate PLGA Prepare priming production protein purification Qiagen receptor recombinant Resuspend room temperature RPMI sample sequence serum solution specific sterile stimulation strategies supernatant syringe T-cell T-cell responses target tion tissue transfected tube tumor vector viral virus vitro vivo Wash µg/mL