DNA Vaccines: Methods and Protocols

Front Cover
W. Mark Saltzman, Hong Shen, Janet L. Brandsma
Springer Science & Business Media, 2006 - 384 pages
In the early 1990s, almost 200 yr after Edward Jenner demonstrated the effectiveness of the smallpox vaccine, a new paradigm for vaccination emerged. The conventional method of vaccination required delivery of whole pathogens or structural subunits, but in this new approach, DNA or genetic information was administered to elicit an immunological response. Once it was observed that plasmid DNA delivered in vivo led to production of an encoded transgene (1), two ground-breaking studies demonstrated that immunological responses could be generated against antigenic transgenes via plasmid DNA delivered by DNA vaccination (as this approach is called) (2,3). The appe- ance of this new vaccination strategy coincided with advances in molecular biology, which provided new tools to study and manipulate the basic elements of an organism's genome and also could also be applied to the design and production of DNA vaccines. DNA Vaccines is a major updated and enhancement of the first edition. It reviews state-of-the-art methods in DNA vaccine technology, with chapters describing DNA vaccine design, delivery systems, adjuvants, current appli- tions, methods of production, and quality control. Consistent with the approach of the Methods in Molecular Medicine series, these chapters contain detailed practical procedures on the latest DNA vaccine technology. The enthusiasm for DNA vaccine technology is made clear by the number of research studies published on this topic since the mid-1990s.

From inside the book

What people are saying - Write a review

We haven't found any reviews in the usual places.

Contents

Design of Plasmid DNA Constructs for Vaccines
11
Vaccination With Messenger
23
A Stress ProteinFacilitated Antigen Expression System
41
In Vitro Assay of Immunostimulatory Activities
55
Delivery of DNA Vaccines Using Electroporation
73
A Brief Overview
83
NeedleFree Delivery of Veterinary DNA Vaccines
91
SurfaceModified Biodegradable Microspheres
107
Modifying Professional AntigenPresenting Cells to Enhance
199
ReplicaseBased DNA Vaccines for Allergy Treatment
221
Immunological Responses of Neonates and Infants
239
DNA Vaccines for Allergy Treatment
253
Protection From Autoimmunity by DNA Vaccination
269
The Use of Bone MarrowChimeric Mice in Elucidating
281
DNA VACCINE PRODUCTION PURIFICATION AND QUALITY
293
Production of Plasmid DNA in Industrial Quantities According
339

Subcellular Trafficking Pathways by Indirect
127
DNA VACCINE ADJUVANTS AND ACTIVITY ENHANCEMENT
137
Complexes of DNA Vaccines With Cationic Antigenic Peptides
159
PrimeBoost Strategies in DNA Vaccines
171
LargeScale Nonchromatographic Purification of Plasmid
351
Assuring the Quality Safety and Efficacy of DNA Vaccines
363
Index
375
Copyright

Other editions - View all

Common terms and phrases

Bibliographic information