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pods in various directions, usually only one being in moi on at a time, while the latter usually extends an armlike pseudopod carrying forward its ectosarc and then proceeding to flow its endosarc with its granular masses and nuclei into this elongated pseudopod, producing a flowing motion within itself, usually in a definite direction, as shown in the illustrations.
For fixing and staining we would recommend the following technic and procedure. The following three methods we have chosen to be best for fixing specimens:
1. A solution suggested by Schaudin, (24) which is made by adding 50 C. C. of absolute alcohol and 5 drops of glacial acetic acid to 100 C. C. of saturated bichlorid of mercury. A wet smear is dropt into this solution for ten minutes. Wash for ten minutes in a little alcohol containing a little iodine and then in 70% alcohol for thirty to forty minutes.
in the iron solution until black clouds cease to be given off. With this method the endosarc is well differentiated, while the ectosarc is recognized by the absence or the stain. Drs. Bass and Johns (20) nave recently suggested a stain for ameba, which can be carried out and examined almost immediately after the specimen is taken, consisting of dipping the specimen in carbofuchsin, which is prepared by dissolving one gram of fuchsin and iu C. C. or absolute alcohol and then add. ing 5 grams of carbolic acid crystals. 10 this mixture add 100 C. C. of distilled water. Leave for 24 hours and filter. The carbofuchsin is immediately washed off and the specimen differentiated in a solution of Loeffler's Methylene Blue. To 30 C. C. of alcoholic solution of Methylene Blue add 100 C. C. of one in one thousand of Aqueous solution of Caustic Potash. The specimen is stained in this solution for one-half minute, washed, dried and examined, preferably with a high power. Another method is that of Giemsa. (27) The specimen must be fixt in either bichlorid or Schaudin's solution. It is then stained for one hour in a freshly prepared solution of 10 drops of Giemsa solution and 10 drops of one per cent of Aqueosus solution of sodium carbonate in 100 C. C. of distilled water. The Giemsa solution is staple and can either be bought or prepared in the laboratory. It is prepared by dissolving 3 grams of Azur II Eosin and .8 of a gram of Azur II in 350 C. C. of 60% methol alcohol. To this solution 250 C. C. of glycerine is added and the whole allowed to stand for 24 hours and then filtered.
2. A method suggested by Dr. Hartzell, which is to drop a wet smear into saturated bichlorid for thirty minutes.
3. Pass an air dried smear thru a flame.
There are also three good methods for staining. In using Heidenhain's Iron Hematoxylin (25) the specimen must be fixed in either of the first two solutions above mentioned. The stain is prepared by dissolving one gram of Hematoxylin crystals in 10 C. C. of hot absolute alcohol and then adding 90 C. C. of water. This solution is allowed to stand in an open, cotton plugged, bottle for four weeks and is then diluted with an equal volume of water before using. The iron solution is made by dissolving 2.5 grams of Ferric Ammonium Sulphate in 100 C. C. of distilled water. The fixt specimen to be stained is first soaked in the iron solution from four to eight hours, then rinsed and immersed in the Hematoxylin from 12 to 24 hours. It is again rinsed and differentiated by immersion
For reference books we would recommend the following: Practical Bacteriology, Microbiol.
ogy and Serum Therapy-A. Besson. Publishers Longmans, Green and Co., New York. Translated by Hutchins
. $11.00 Pathogenic Micro organisms-Mac
Neal, Publishers P. Blakiston's
Pathogenic Bacteria and Protozoa -McFarland. Publishers W. B. Saunders and Co., Philadelphia, Pa.
General Bacteriology—Jordan. Publishers W. B. Saunders and Co., Philadelphia, Pa. We would strongly urge those members of the dental profession, who have the facilities and interests in the most rapid furtherance of mouth infection studies, to secure a microscope, which need not be an expensive one, but equipped with a highpower dry objective and each a high and a low power eye piece, and some good reference books, and proceed to make observations and records, both for the great advantage of understanding and interpreting the conditions of their own patients as they present themselves and that they may contribute their collected findings to the sum total of data. It is only by collective and earnest study that the contradictions and evidence previously herein recited can be resolved into simple statements of facts, for truths do not conflict or contradict, tho partial truths and misread conditions do.
1. The term endameba is used in preference to ameba, as pointed out by Williams' "Pathogenic Micro-organisms." p. 155. The endameba is parasitic for man and the ameba a free living saprophyte. Endameba is preferred to entameba, as pointed out in a note by Bass & Johns, as suggested by Smith in J. A. M. A., Vol. 64, No. 7, p. 19. 2. Prowazek-reference-Arbeit aus dem kaiserlich Gesundheitz amte. 1904 Vol. XXI, p. 42. 3. Sternberg-reference-Leitschr F. Gegewartige medizin 1862, Nos. 21-24.
4. Grasse-reference-Die protozoi parassiti e specialmente diquelti che sono nell uonro-Gazzetía medica italiana 1879.
Gros-reference-Cit leg neveer Lemaire 147.
7. Chiavaro-Dental Review-Vol. 28, No. 12, p. 1122.
8. Die Protozoa als Frank Kranklieitserreger Jena 1901, p. 30.
9. See Jordan, "General Bacteriology."
Barret-Dental Cosmos 54-No. 8, p. 949.
11. Skilled Bacteriologists Affirmative. See 14. 12. McFarland-Pathogenic Bacteria and Pro
13. Amesse J. A. M. A., Vol. 63, No. 21, p. 1811.
Barret-Dental Cosmos 54, No. 8, p. 949.
Smith, Middleton & Barrett, J. A. M. A.,
Johns-J. A. M. A., Vol. 64-No. 7, p. 553.
Bass & Johns-N. O. M. & S. J., Vol. 67,
Howe, D. Cosmos, Vol. 57, No. 3, p. 307, J. A. D. S., Vol. 19, No. 14, p. 584.
16. Files of Commission.
17. Curet-N. O. M. & S. J., Vol. 67, No. 5, p. 464.
Wherry J. Inf. Dis. 1912-10-162-165.
Rogers Brit. Med. J. 1912, 1424.
Smith, Middleton & Barrett, J. A. M. A., Vol. 63, No. 20, p. 1746.
21. White-D. Cosmos, Vol. 57, No. 4, p. 405. 22. Noguchi, J.-Exp. Med., Vol. 16. 23. Jordan-"General Bacteriology," p. 158. 24. Besson-Practical Bacteriology, Micro-biology and Serum Therapy, 749.
Mac Neal "Pathogenic Micro-organisms,
26. Bass & Johns-J. A. M. A., Vol. 64, No. 7, p. 553.
27. Besson-"Practical Bacteriology," Microbiology and Serum Thearpy."-p. 727.
IMPORTANT NOTICE REGARDING
The Research Department is now receiving, regularly, tungsten and molybdenum of splendid quality and can furnish, immediately, or on short notice, these materials in almost any quantities for thoro testing and practical uses. Special diamond dies have been constructed for drawing the metal in the larger sizes in splendid quality. Application forms for the materials can be secured from the chairman.
As we are furnishing these materials at cost, we will ask you to kindly include postage and registration or insurance fee, as well as the amount for the metal.
RESEARCH FUND REPORT.
We, herewith, publish in detail the amounts of cash and subscriptions received to date from the various states and their chief cities. This report includes only the cash and pledges that have reached the Chairman by April 15, 1915. Several states have reported more than is shown in these figures but the signed subscriptions, or their equivalent, have not yet been received.
In many communities only one payment is due, while in others two and three, which, in a large part, accounts for the difference in the ratio of payments to pledges. Several states are planning to take the wo up at their spring and summer meeting. The total cash and subscriptions to date,
April 15, 1915, for which the pledges are in hand, are......
. $48,692.43 The total cash received to date.
12,312.50 (Including $1079.00, organization ex
pense contributed by Chairman.) The researches of last year cost, per report in October issue.
4,378.06 Organization expense to Jan. 1, 1914.. 1,079.00 The budget for this year is.
8,000.00 Balance due to meet this year's budget.. 1,144.66
This money is being used for paying technicians, for laboratory expenses, etc. No money is paid to the various directors of researches. The seemingly imperative research work that we must push next year is so urgent that our budget must, if possible, be double what it is this year. This will be possible if some of the states that have not acted as yet do so promptly. Those who have made subscriptions will see the necessity of making their payments promptly as they come due.
The following is a detailed statement:
701$2430 00 $757 00
001 00 0
001 00 0 00
00 185 00 46 00
192 $3917 50 $405 50
3 67 50 6 00 41 1240 001 70 00 131 2180 00 307 50
The above report is being modified from day to day as subscriptions are being received and we shall be very glad for any suggestions or corrections.
WESTON A. PRICE, Chairman.
82 $1105 001 $362 00
615 00 164 00