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Endameba buccalis. Unstained living organism, showing its motility and progressive changes of

pseudopods as recorded at half second periods for twelve seconds. (See text.)

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species predominating may vary with referred to later. Its size varies from 6 the location and the time of the year, to 30 microns (one four thousandth to in the

mouth under certain one eight hundredth of an inch in diamconditions, as indicated later. Fig. 1 eter.) When active it throws out a few shows a typicai endameba (1) buccalis of lobose pseudopods, which have a definite Prowazek (1904) (2) which is probably independent tho consecutive movement the same as endameba buccalis of Stern- which is clearly shown in these unberg (1862), (3) the ameba dentalis of stained illustrations from life. This Fig.

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Endameba buccalis.

Illustrating nuclei, ingested matter and bacteria, also at A and B the

ectoplasm and ectosarc. Half second intervals.

a

are downward, first toward the right and then toward the left. In the third row the upper border has changed but little, but the thrust is farther towards the left and in the fourth row, while the lower border remains about constant, the motion has been exerted again successively more and more in an upward direction. During this time, the endosarc, including the granular mass within has changed relatively little as shown by the location of its nuclei and ingested material. The red blood corpuscles shown in the three upper rows give a good conception of the relative size of the endameba. With this species the pseudopod is sel

short blunt pseudopod with its limiting membrane, and letter A, its glassy hyaline ectoplasm. They have slightly yellowish tint, giving a color between that of a leukocyte and a red blood cell. Frequently in extra good specimens the ectosarc with its contained protoplasm seems to be relatively large in proportion to its contained granular endosarc and gives the appearance that the latter is floating within it. In these specimens there are usually several very large blunt pseudopods present at once, tho note, as previously stated, only one is motile at a time. These characteristics are shown especially well in Fig. 3.

It frequently occurs that it is exceed- Its form may vary from a narrow worm ingly difficult to differentiate external like structure to nearly or actually foreign matter from the structural parts round, according to the conditions. When of the endamebae, because of their being motile it projects a pseudopod in the superimposed. This is particularly well direction it desires to move and graduillustrated in Fig. 4 where, by knowing ally extends this until its ectosarc is in that this species, endameba buccalis, does the form of a long loop with its refrac

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Endameba buccalis. Showing differentiation of endosarc and successive, but single projections of

pseudopods. Half second intervals.

not extend long pseudopods, as this view would seem to indicate we would recog. nize that the apparent pseudopod is an erythrocyte and some adjoining foreign material. The apparent effect of this is that of a slowly extending pseudopod.

The other common species is known as endameba Kartulisi, discovered by Kartulis (8) in Egypt, the morphology of which is distinctly different from the preceding when seen in its natural state, but which, according to the manner of its dying, may take on forms quite unnatural to it in life and which more closely resemble the endameba buccalis.

tile protoplasm practically all in advance of the granular mass.

Figure 5 shows an endameba of this species, starting out on a journey, as seen in the first picture in the upper left. You will note that the condition just spoken of clearly obtains. In the second picture, one-half second later, we see the granular mass flowing within the pseudopod. Unlike the species buccalis, just described, this variety continues its projection almost entirely in one direction, which gives us the effect of a repeatedly extended single pseudopod. It will be noted that at the time the granular mass

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Endameba buccalis. Showing relative size to a red blood cell, also a superimposition with for

eign material giving the false appearance of a pseudopod. Half second intervals.

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Endameba Kartulisi. Showing organism starting on a journey and its progress in six seconds.

Half second intervals.

because of the migratory nature and habit of the organism. Pictures 4 and 5 illustrate how the least advanced end of the organism has scarcely started to move or change its contour until the mass

and has practically doubled in length. It should be noted that the organism covers nearly twice the square surface in the last of this series as in the first. This may be due, in a large part, to a

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See text.

Endameba Kartulisi. Showing nuclei and surrounding nourishing vacuoles A and B.

Half second intervals.

how endameba reproduce, but it is gen- maximum distance apart and the 5th, erally supposed that it is by transverse one-half second later, they are close todivision and by some it is contended that gether. This motion is very spectacular they do so by means of division between when seen in the motion pictures, for in the double nuclei. (9) It is significant to each of these series the intervening note from these illustrations, as shown steps, covered by seven pictures, have particularly in figure 6, the clearly de- been left out. Figure 6 also illustrates fined double nuclei, which clearly dif- the cumbersomeness of the organism, as ferentiates this species from the shown by the difficulty it has in changhistolytica found in the colon, which ing its course. In the last picture of the multiplies by fragmentation of the upper row it has decided to change its chromatin, the remnant of the nuclei direction and it moves the mass of its itself being expelled from the cyst. Fig. body much as a company of soldiers ure 6 also shows very beautifully, and would be marched around a corner, selwhich often cannot be seen in stained dom cutting the corners. The halo surspecimens, that the double nuclei are not rounding these nuclei, shown with par

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